FIG 6.

The E3 ligase activity and the integrity of the C-terminal parts of TRIM56 are essential for the antiviral activity against YFV. (A) Immunoblot analysis of HA-tagged wild-type and mutant TRIM56 and YFV NS3 protein expression in 293-FIT-T56 WT or Mut cells in the absence (odd-numbered lanes) or presence (even-numbered lanes) of Tet and mock infected or infected with YFV-17D for 24 h (MOI = 0.1). There were eight lines of 293-FIT-T56 WT or Mut cells examined in total, data from which are shown in eight blocks of immunoblot collections. (B) Progeny virus production in culture supernatants of 293-FIT-T56 WT or Mut cells cultured in the absence or presence of Tet at 24 h after infection with YFV-17D (MOI = 0.1).