FIG 7.

The suppression of DENV2 multiplication by TRIM56 requires its E3 ligase activity and the integrity of the C-terminal portions. (A) Immunoblot analysis of HA-TRIM56 (WT or mut) and DENV2 prM protein abundance (using goat anti-DENV2 antibody) in 293-FIT-T56 WT or individual Mut cells in the absence (odd-numbered lanes) or presence (even-numbered lanes) of Tet and mock infected or infected with DENV2-16681 (MOI = 1) for 60 h. (B). Progeny virus production in culture supernatants of 293-FIT-T56 WT or Mut cells cultured in the absence or presence of Tet at 60 h after infection with DENV2-16681(MOI = 1). Single and triple asterisks indicate that statistical differences exist between −Tet and +Tet cells with P values of <0.05 and <0.001, respectively.