FIG 9.

The inhibition of HCoV-OC43 propagation by TRIM56 is dependent upon its E3 ligase activity but not upon the integrity of the C-terminal portions or the B box or coiled-coil domain. (A) Immunoblot analysis of HA-TRIM56 (WT or Mut) and HCoV-OC43 N protein (N) abundance in 293-FIT-T56 WT or individual Mut cells in the absence (odd-numbered lanes) or presence (even-numbered lanes) of Tet and mock infected or infected with HCoV-OC43 (MOI = 0.1) for 24 h. (B) Progeny virus production in culture supernatants of 293-FIT-T56 WT or Mut cells in the absence or presence of Tet at 24 after infection with HCoV-OC43 (MOI = 0.1).