FIG 2.

Quantitative assay of the HERV-K(HML2) Env-Rec-mediated inhibition of Tetherin activity. (A) Schematic description of the experimental procedure. 293T cells were transfected with the indicated plasmids. Three days posttransfection, the supernatants of producer cells were harvested to infect target cells. The percentage of infected cells was measured after 72 h by flow cytometry analysis, and viral titers were calculated for each condition. (B) The evolution of viral titers with the amount of transfected Tetherin (0 to 100 ng) is plotted for each potential inhibitor. The 100% value was set for each inhibitor using the titer measured without Tetherin. Error bars represent standard deviations of the means from 10 independent experiments, except for HIV-1 Vpu data (3 experiments).