FIG 2.

C. elegans is not preferentially attracted to the WT or UTI89 csgBA bcsA mutant. Cells of WT UTI89 or the csgBA bcsA mutant were spotted on opposite sides of a YESCA-cholesterol agar plate. After 2 days of biofilm development at 26°C, C. elegans myo-2::rfp ceh22::gfp worms were moved onto the center of the plate (A). Shown is a representative bright-field/Texas Red overlay of WT or csgBA bcsA colonies with fluorescent worms after 6 h of C. elegans exposure (B). At 1, 6, and 24 h post-C. elegans addition, fluorescent images were taken, and the numbers of worms on each strain were counted (C). Worm totals for each plate at each time point fell between 200 and 1,200. Five individual plates were averaged, and bars represent the number of worms on each strain divided by the total number of worms counted on that particular plate. Error bars represent standard deviations.