FIG 2.

Gel mobility shift assays using a labeled DNA fragment containing the wblA_ch promoter region and the His₆-AdpA_ch protein. Lane 1, labeled fragment; lanes 2 to 5, labeled fragment with 0.05 μg, 0.1 μg, 0.2 μg, and 0.4 μg of purified His₆-AdpA_ch protein, respectively; lane 6, labeled fragment with a 100-fold excess of nonspecific DNA and 0.2 μg purified His₆-AdpA_ch protein; lane 7, labeled fragment with a 100-fold excess of unlabeled wblA_ch promoter DNA and 0.2 μg purified His₆-AdpA_ch protein.