FIG 3.

DNase I footprinting assay for determination of the AdpA_ch-binding site. (A) A 5′-FAM-labeled wblA_ch probe was used in the DNase I footprinting assay without or with purified AdpA_ch (10 μg). The protected regions are underlined. (B) Sequences of the determined AdpA_ch-binding sites and the consensus AdpA_ch-binding sequence (in boldface type). (C) Nucleotide sequences of the wblA_ch promoter region and the predicted AdpA_ch-binding sites. The transcription start point (tsp) is marked by a bent arrow, the AdpA_ch-binding sites are overlined, and the ribosome-binding site (RBS) is boxed.