TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| S. chattanoogensis | ||
| WT | S. chattanoogensis L10 wild type | 30 |
| ΔwblAch | wblAch-disrupted mutant | This study |
| ΔadpAch | adpAch-disrupted mutant | 28 |
| YC1 | wblAch-disrupted mutant complemented with wblAch and its own promoter | This study |
| YC2 | Wild-type strain carrying wblAch overexpression | This study |
| YC3 | Wild-type strain carrying an empty vector | This study |
| Saccharomyces cerevisiae BY4741 | Indicator organism for natamycin bioassays | Laboratory stock |
| E. coli | ||
| TG1 | General cloning host | Novagen |
| ET12567/pUZ8002 | Methylation-deficient E. coli strain for conjugation with the helper plasmid | 41 |
| BL21(DE3) | Host for protein expression | Novagen |
| BW25113/pIJ790 | Strain used for PCR-targeted mutagenesis | 27 |
| Plasmids | ||
| pMD19-T vector | General cloning vector | TaKaRa |
| pT-wblAch | pUC18 containing the promoter of wblAch | This study |
| pSET152 | Integrative shuttle vector | 26 |
| pIJ8630 | Integrative shuttle vector | 42 |
| pIJ773 | Source of apramycin resistance cassette; aac(3)IV oriT | 27 |
| pMRD312 | Cosmid containing wblAch | This study |
| pMRD313 | wblAch replaced by aac(3)IV cassette in pMRD312 | This study |
| pYP1 | wblAch replaced by an 81-bp scar in pMRD312 | This study |
| pYP2 | pSET152 carrying wblAch and its promoter | This study |
| pYP3 | pIJ8630 carrying wblAch and ermE* promoter | This study |