FIG 3.

Analysis of plasmids of C. botulinum type A(B) isolates. (A) Plasmid DNA fractions extracted from 7 type A(B) strains were analyzed with 0.8% agarose gel electrophoresis. Arrowheads indicate positions of plasmidlike DNAs. (B) HindIII digestion pattern of plasmidlike DNA from Ibaraki2007 (pIB07). (C) Structure of the pIB07 plasmid. The positions of the 2 HindIII sites are indicated. Arrows indicate 5 genes larger than 150 bp. These genes were annotated as follows: 1, replication initiation protein; 2, putative lipoprotein; 3, hypothetical protein; 4, hypothetical protein; and 5, plasmid recombination enzyme.