FIG 4.

Detection of 21-kb plasmid predicted by genome sequencing. (A) Strategy of PCR scanning for plasmid detection. Six double-headed arrows indicate amplicons that cover the whole plasmid sequence. Sizes of amplicons are shown. PCR primer pairs used for the analysis are listed in Table 2. (B) Results of PCR scanning. Genomic DNAs from type A(B) C. botulinum strains were used for the template. PCR products of the six amplicons were analyzed by 0.8% agarose gel electrophoresis. Names of strains analyzed are shown above the gels. Lane numbers correspond to amplicon numbers. Lanes M, 1-kb-ladder DNA size markers.