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. 2014 Nov;80(22):7061–7070. doi: 10.1128/AEM.02207-14

FIG 2.

FIG 2

Purification, functional expression, and characterization of the recombinant GOR. (A) SDS-PAGE (12%) of the crude extract (lane 1) and HisTrap-purified proteins (lane 2). Twenty-five micrograms of protein was loaded per well. (B) Glutathione reductase activity of the recombinant protein in the presence of 0 or 2.5 mM GSSG. (C to E) Effect of pH (C), temperature (D), and ionic force (E) on the TR activity of the recombinant GOR. Bars represent the averages of three independent trials ± standard deviations.