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Purification of tellurite reductase activity from BNF22
| Step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Purification (fold) |
|---|---|---|---|---|
| Crude extract | 662 | 74,200 | 112 | 1 |
| DEAEa | 63 | 60,633 | 960 | 9 |
| Rotofor 1 | 12 | 38,665 | 3,171 | 28 |
| Rotofor 2 | 2 | 13,683 | 6,655 | 59 |
| UF 50 kDa | 1.6 | 16,235 | 9,945 | 89 |
Phosphocellulose P11 column linked in tandem to a DEAE-Sepharose column. See Materials and Methods for details.
