FIG 7.

cis-trans isomerase (CTI) expression affects Pseudomonas membrane hydrophobicity. (A) Relative mRNA expression levels of CTI (PA1846) were assessed for 24-h static cultures of PAO1 and ΔmodA strains, corrected for 16S rRNA abundance. (B) The bacterial-adherence-to-hydrocarbons (BATH) assay was performed using xylene. Changes in membrane lipid hydrophobicity are reflected in altered adherence to xylene. The “+ NO₃” notation indicates that cultures were grown with 100 mM KNO₃. Data represent the means ± standard errors of the means, where n is ≥3 independent biological replicates. Statistical significance was determined using a two-tailed unpaired Student t test, where * represents a P value of <0.05 and *** represents a P value of <0.005.