FIG 5.

The biosynthesis pathways of RL, Psl, and Pel have the sugar precursor Glc-1-P (catalyzed by AlgC) in common. (A) Schematic representation of the P. aeruginosa PAO1 metabolic routes for the biosynthesis of Psl, Pel, and RL. The solid lines represent known metabolic routes, and the dashed line represents a hypothetical route. (B) Swarming zone of PAO1/algC (algC was constitutively expressed by plasmid pLPS188.) and control strain PAO1/vector. (C) Comparison of RL production in PAO1/algC and PAO1/vector. The amount of RL was normalized to the PAO1/vector level (261 mg/liter). (D) Comparison of PAO1/algC and PAO1/vector Psl levels. *, P < 0.05.