TABLE 1.
Bacterial strains and plasmids used in this study
| Stain or plasmid | Description/genotypea | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| NEB 5-α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 ϕ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | New England BioLabs |
| BL21(DE3) | F− ompT gal dcm lon hsdSB(rB− mB−) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Merck, Millipore, Novagen |
| Plasmids | ||
| pJET1.2 | Ampr; cloning plasmid | Thermo Fisher Scientific |
| pET15b | Ampr; expression plasmid | Merck, Millipore |
| pIZ1036 | Kmr; broad-range expression plasmid | 42 |
| pIBA34 | pIZ1036 carrying phnA3 and phnA4 from strain CHY-1 | 18 |
| pJCA1 | pJET1.2 carrying pahAb | This study |
| pJCA3 | pJET1.2 carrying pahAa | This study |
| pJCA5 | pJCA3 carrying pahAb from pJCA1 downstream of pahAa | This study |
| pJCA6/pJCA7 | pJET1.2 carrying two different pahAc5-pahAd5 amplicons | This study |
| pJCA8/pJCA9 | pJET1.2 carrying two different pahAc4-pahAd4 amplicons | This study |
| pJCA10/pJCA11 | pJET1.2 carrying two different pahAc2-pahAd2 amplicons | This study |
| pJCA13/pJCA14 | pJET1.2 carrying two different pahAc8-pahAd8 amplicons | This study |
| pCAE1 | pET15b carrying pahAc2-pahAd2 from pJCA10 | This study |
| pCAE4 | pET15b carrying pahAc8-pahAd8 from pJCA14 | This study |
| pCAE5 | pET15b carrying pahAc4-pahAd4 from pJCA9 | This study |
| pCAE7 | pET15b carrying pahAc5-pahAd5 from pJCA7 | This study |
| pCAZ1 | pIZ1036 carrying the pahAa-pahAb insert from pJCA5 | This study |
a
Ampr, ampicillin resistance; Kmr, kanamycin resistance.