TABLE 2.
Oligonucleotides used for PCR amplification of RHD genes
| Designation | Sequence (5′–3′)a | Targeted gene(s) |
|---|---|---|
| C095-F | GAAGGAGATATATTATGGATAATAATTTCACCATC | pahAa |
| C095-R | CGTCGACTTAATAAAAACGGTCAAAATGC AACTG | pahAa |
| C113-F | GCTCGAGGAGATATTGTATGAATACCCGTGTCAA | pahAb |
| C113-R | CTTATTCTACTTCTGCCTCAA | pahAb |
| C951-F | GCATATGATAAATATAGATGATCTGATTGA | pahAc2-pahAd2 |
| C341-Rb | CTCGAGTTAAAATAAAGTGTTCATGTTGCTATC | pahAc2-pahAd2 |
| C763-F | GCATATGATGAAGCCAAGCGAGTTGATTGA | pahAc4-pahAd4 |
| C569-F | GCATATGGTCGATGTAAACAGTCTG | pahAc3-pahAd3 |
| C2271-R | GCCTACCCAATGGCTGATGCC | pahAc3-pahAd3 |
| C451-F1 | GAAGGAGATATCATATGAATGAATGGCTGGAGGAG | pahAc5-pahAd5 |
| C451-R | GTCTAGATCAGAAAAACATATTCAGATTTTTATC | pahAc5-pahAd5 |
| C451-F2*c | GAAGGAGATATCATATGAAAAACATTAACTATCAGGAAC | pahAc5-pahAd5 |
| JCA7-R2* | GGCTCGAGATCAGAAAAACATATTCAGA | pahAc5-pahAd5 |
| C5241-F | GCATATGTTCGATATCAAGAATTTAATCAA | pahAc8-pahAd8 |
| C427-R2 | CTCGAGTTACAAGATAAACAACAAGTTTTTCCC | pahAc8-pahAd8 |
a
Letters in italics indicate restriction sites for one of the following enzymes: NdeI, SalI, XhoI, or XbaI.
b
This primer was used for the amplification of pahAc4-pahAd4, together with C763-F.
c
Primers used for subcloning pahAc5-pahAd5 in pET15b are indicated by an asterisk (*).