TABLE 3.
Affiliation of 16S ribosomal sequences identified in the metagenomic data set obtained from labeled DNA
| Proposed affiliation | Contig | Length (bp) | Best-match GenBank accession no. | Query cover (%) | Identity (%) | Relevant OTUa |
|---|---|---|---|---|---|---|
| Acidobacteria | ||||||
| Unclassified | 815 | 1,275 | FQ659841 | 100 | 96 | OTU397 |
| Unclassified | 9991 | 531 | JN178653 | 67 | 97 | NF |
| Alphaproteobacteria | ||||||
| Bradyrhizobiaceae | 6706 | 598 | JN869027 | 86 | 94 | NF |
| Betaproteobacteria | ||||||
| Rhodocyclaceae | 6757 | 600 | FQ660427 | 100 | 99 | OTU17 |
| 8969 | 551 | FQ660504 | 100 | 100 | OTU101 | |
| Burkholderiales | ||||||
| Comamonadaceae | 10394 | 520 | FQ660439 | 100 | 98 | OTU6 |
| 10648 | 519 | FQ659005 | 100 | 99 | OTU154 | |
| Gammaproteobacteria | ||||||
| Unclassified | 1848 | 929 | FQ660401 | 100 | 99 | OTU2 |
| Sinobacteriaceae | 2378 | 848 | FQ660299 | 100 | 99 | OTU153 |
| Xanthomonadaceae | 3372 | 740 | JN868994 | 87 | 93 | NF |
| Xanthomonadales | 8678 | 548 | EF632898 | 67 | 97 | |
| FQ658754 | 38 | 96 | OTU153 |
a
That is, the OTU previously associated with phenanthrene degraders as deduced from SIP experiments on the same study site (7). NF, no relevant OTU found.