FIG 1.

Schematic representation of lacZ translational fusion (A) and lacZ transcriptional fusion (B). (A) In the construct shown, the transcription of ′lacZ is driven by the x promoter (P) and the mRNA produced is translated from the SD sequence of x mRNA to produce a hybrid protein with several N-terminal amino acids of X and a large functional C-terminal fragment of β-galactosidase. The translational fusion data can reflect the strength of the promoter and the efficiency of initiation of translation of the target gene. (B) In the construct shown, the transcription of lacZ is driven by the x promoter and the mRNA produced is translated by the SD sequence of lacZ mRNA to produce a wild-type β-galactosidase. The transcriptional fusion data can reflect the strength of the promoter of the target gene.