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. 2014 Nov;80(21):6627–6637. doi: 10.1128/AEM.02184-14

FIG 2.

FIG 2

Effect of gacA mutation on lipA expression. (A) Influence of a gacA mutation on expression of a chromosome-borne lipA′-′lacZ translational fusion and growth of the bacteria. β-Galactosidase activity (solid symbols) and growth (open symbols) were measured in the wild-type strain (Pf-5F3; squares) and the gacA mutant (Pf3563F3; circles) at various time points after inoculation into 50 ml LB medium. (B) Influence of the gacA mutation on expression of a chromosome-borne lipA′-′lacZ translational fusion (wild-type strain, Pf-5F3; gacA mutant, Pf3563F3) and a chromosome-borne lipA-lacZ transcriptional fusion (wild-type strain, Pf-5F4; gacA mutant, Pf3563F4). The β-galactosidase activity of each strain was measured in the stationary phase after inoculation into 50 ml LB medium. (C) RT-PCR analysis of lipA expression in the wild-type strain (Pf-5; lane 1) and the gacA mutant (Pf3563; lane 2). (D) Relative whole-cell lipase activity in the wild-type strain (Pf-5) and the gacA mutant (Pf3563). All experiments were performed in triplicate, and the mean values ± standard deviations are indicated. *, P < 0.05 compared with the control.