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. 2014 Nov;80(21):6627–6637. doi: 10.1128/AEM.02184-14

FIG 5.

FIG 5

Effects of rsmA and rsmE overexpression on lipA expression. (A) Influences of rsmA and rsmE overexpression on expression of a chromosome-borne lipA′-′lacZ translational fusion and growth of bacteria. β-Galactosidase activity (solid symbols) and growth (open symbols) were measured in the wild-type strain (Pf-5F3/pBBRKm; squares), an rsmA-overexpressing strain (Pf-5F3/pBBRKrsmA; triangles), and an rsmE-overexpressing strain (Pf-5F3/pBBRKrsmE; circles) at various time points after inoculation into 50 ml LB medium. (B) Influences of rsmA and rsmE overexpression on expression of a chromosome-borne lipA′-′lacZ translational fusion (bar 1, wild-type strain Pf-5F3/pBBRKm; bar 2, rsmA-overexpressing strain Pf-5F3/pBBRKrsmA; bar 3, rsmE-overexpressing strain Pf-5F3/pBBRKrsmE) and a chromosome-borne lipA-lacZ transcriptional fusion (bar 4, wild-type strain Pf-5F4/pBBRKm; bar 5, rsmA-overexpressing strain Pf-5F4/pBBRKrsmA; bar 6, rsmE-overexpressing strain Pf-5F4/pBBRKrsmE). The β-galactosidase activity of each strain was measured in the stationary phase after inoculation into 50 ml LB medium. (C) RT-PCR analysis of lipA expression in wild-type strain Pf-5/pBBRKm (lane 1), rsmA-overexpressing strain Pf-5/pBBRKrsmA (lane 2), and rsmE-overexpressing strain Pf-5/pBBRKrsmE (lane 3). (D) Relative whole-cell lipase activity in wild-type strain Pf-5/pBBRKm, rsmA-overexpressing strain Pf-5/pBBRKrsmA, and rsmE-overexpressing strain Pf-5/pBBRKrsmE. All experiments were performed in triplicate, and the mean values ± standard deviations are indicated. *, P < 0.05 compared with the control; **P < 0.01 compared with the control.