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Effects of mutations in the SD sequence of lipA mRNA on translational repression by RsmE
| Plasmid | β-Galactosidase activity (Miller units) for strain: |
Repression factor | |
|---|---|---|---|
| BL/pET-28a | BL/pET-rsmE | ||
| pBBR003 | 1,312.5 ± 106.4 | 818 ± 75.3 | 1.60a |
| pBBR00A10U | 1,323.9 ± 110.8 | 1,018.6 ± 95.4 | 1.30a |
| pBBR00G11C | 611.5 ± 56.9 | 541.2 ± 42.8 | 1.13 |
| pBBR00G12A | 712 ± 69.5 | 608.5 ± 60.7 | 1.17 |
| pBBR00A14U | 1,025.6 ± 101.4 | 967.5 ± 98.3 | 1.06 |
P < 0.05 compared with the control.
