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. 2014 Nov;80(21):6638–6646. doi: 10.1128/AEM.02289-14

FIG 3.

FIG 3

(A and B) Total U immobilized (gray bars) and fraction reduced to U(IV) (open bars) by WT biofilms grown for 24, 48, and 72 h (A) and by 48-h-old biofilms of the pilin-deficient pilA mutant and the hyperpiliated pilA+ strain (B). The biofilms were exposed to 1 mM uranyl acetate for 24 h. Shown are averages and standard deviations for triplicate biofilm samples (WT) and averages and standard errors for duplicate biofilm samples (pilA and pilA+ strain). Significant differences in t-test pairwise comparisons with the 24-h-old (A) or 48-h-old (B) WT biofilms are indicated: *, P < 0.05; **, P < 0.005. (C) Heme-stained SDS-PAGE of protein extracted from the EPS matrix of 48-h-old biofilms of the WT, pilA, and pilA+ strains. Numbers at the right are the relative molecular masses of protein markers (in kDa). The arrow points to the migration of the small, processed form of the OmcZ c cytochrome (OmcZS).