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A polysaccharide stain on thin sections of high-pressure-frozen and freeze-substituted cells of the mesophilic verrucomicrobial methanotroph strain 4AC indicates that polysaccharides are present in the electron-light particles. Electron-light particles, appearing as white spots in the negative control (B), show clear staining in panel A (black silver aggregates) because of the polysaccharide content. Similar results were obtained for strains 3B and 3C (data not shown).
