TABLE 1.
Primers and probes targeting the investigated viruses in sewage and patient samples
| Virus | Sequence of: |
Reference or source | ||
|---|---|---|---|---|
| Forward primer | Reverse primer | Probe | ||
| Norovirus GI | YTGGATGCGNTTYCATGA | CTTAGACGCCATCATCATT | AGATYGCGATCYCCTGTCCAAGATCGCGGTCTCCTGTCCA | Modified from reference 73 |
| Norovirus GII | TGGAYTTTTAYGTGCCCAG | CGACGCCATCTTCATTCAC | AGCCAGATTGCGATCGCCC | 74 |
| Astrovirus | TCTTCTCAACGTGTCCGTATCTAATCAACGTGTCCGTA | TTTTGGTCCTGTGACACC | ATTGTCAATAARCAACTCAGGAACA | This study |
| Adenovirus | CTCGACATGACTTTTGAGGT | GTAGACGGCCTCGATGAC | AGCCCACACTTCT | Modified from reference 75 |
| Aichi virus | TGGGCTGCCATGTACAA | GAGCCGTTGACGGTCAC | CACCCACTCCATGTGGAATTGC | This study |
| HAV (qPCR) | AAAGTGGAGTTTASTCAGTGTAAAGTGGAATTYACTCAGTGTAAAGTGGAATTTACTCAATGT | ACWGCAGGAAAATTAATCATG | TGAATGTGGTCTCCAAAACGCTGAATGTAGTCTCCAAAACGC | This study |
| Parechovirus | ATGGCGTGCCATAACTCTAATGGCGTGCCATAATTCTA | AGAGGCACCTGTTACCAGAGGGGCACCTGTTACCACAGAGGCATCTGTTACCAG | CTTATGCTCACACAGCCATCCTCT | This study |
| Rotavirus | ACCATCTACACATGACCCTCACCATCTTCACGTAACCCTC | GGTCACATAACGCCC | ATGAGCACAATAGTTAAAAGCTAACACTGTC | Modified from reference 76 |
| HEV | GGTGGTTTCTGGGGTGACACGCGGCACACGATCAG | AGGGGTTGGTTGGATGAAGGGGTTGGTTGGGTGAA | TGATTCTCAGCCCTTTGATTCCCAGCCCTT | This study |
| HAV (PCR for sequencing first PCR) | AAYGTTGCTTCYCATGTYAGRGT | ACCADGCCATDCCATCHACATC | 77 | |
| HAV (nested PCR) | ATGYTTTGCWCCHCTHTATCAT | ACCADGCCATDCCATCHACATC | 77 | |