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Design of yeast surface display for assembly of minicellulosomes. (A) Schematic representation of the three-enzyme strain. In this conventional minicellulosome system, EG2, CBH2, and BGL1 are secreted and docked onto the cohesin scaffolds to form trifunctional minicellulosomes. (B) Schematic representation of the five-enzyme strain. On top of the three cellulases, LPMO and CDH are secreted and docked to enhance cellulose degradation and utilization.
