TABLE 1.

Recombinant S. cerevisiae strains used in the cellulose hydrolysis studies

Strain	Plasmid(s)	Phenotype
Control (EBY100)	pYD1-CipA3 and pRS425-insert	Galactose-inducible display of three-domain miniscaffoldins without cellulase activity
Three-enzyme minicellulosome (EBY100)	pYD1-CipA3-EG2 and pRS425-CBH2-BGL1	Galactose-inducible display of trifunctional minicellulosomes with CBH2, EG2, and BGL1 activities
Five-enzyme minicellulosome (EBY100)	pYD1-CipA5-EG2-LPMO-CDH and pRS425-CBH2-BGL1	Galactose-inducible display of pentafunctional minicellulosomes with CBH2, EG2, BGL1, LPMO, and CDH activities
Three-enzyme minicellulosome CipA5 (EBY100)	pYD1-CipA5-EG2 and pRS425-CBH2-BGL1	Galactose-inducible display of trifunctional minicellulosomes with CBH2, EG2, and BGL1 activities
Control (HZ848)	pRS426-control	Control, no display
Three-enzyme minicellulosome (HZ848)	pRS426-CipA3-CBH2-EG2-BGL1	Constitutive display of trifunctional minicellulosomes with CBH2, EG2, and BGL1 activities
Five-enzyme minicellulosome (HZ848)	pRS426-CipA5-CBH2-EG2-BGL1-LPMO-CDH	Constitutive display of pentafunctional minicellulosomes with CBH2, EG2, BGL1, LPMO, and CDH activities