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Nup205 is required for nuclear localization of HIV Rev but not E4orf4. (A) Western blot demonstrating knockdown of Nup205 in HeLa cells. A tubulin blot is included as loading control. (B) Epifluorescence microscopy showing localization of EGFP-fused SV40-LgT, E4orf4, and HIV Rev in HeLa cells after transfection with nonsilencing or Nup205 siRNAs.
