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. 2014 Nov;88(22):13249–13259. doi: 10.1128/JVI.00933-14

FIG 8.

FIG 8

E4orf4 and Nup205 regulate viral gene expression. (A) Western blot showing expression of indicated viral proteins at various hours postinfection (h.p.i) in H1299 cells infected with wild-type (H5pg400) Ad5 or the E4orf4− virus. (B) Western blot showing expression of viral proteins at indicated h.p.i. in H1299 cells transfected with nonsilencing or siRNA targeting Nup205. (C) Western blot demonstrating knockdown of Nup205 in H1299 cells. A tubulin blot is included as loading control. (D) Quantitative RT-PCR of late viral gene expression (mRNAs with tripartite leader) at 23 hours postinfection in H1299 cells transfected with nonsilencing or siRNAs targeting Nup205. (E) qRT-PCR of early gene expression (E1A and E4orf6) at 7.5 hours postinfection.