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Knockdown of Nup205 impairs viral replication and delays the cytopathic effect. (A) Replication of wild-type Ad5 (H5pg400) in H1299 cells transfected with nonsilencing or two separate siRNAs targeting Nup205. (B) Light micrographs showing appearance of cells infected with wild-type Ad4 under conditions of Nup205 knockdown or control. (C) Western blot demonstrating knockdown of Nup205 in H1299 cells. A tubulin blot is included as a loading control.
