FIG 2.

The ZIP domain of HBZ is required for transcriptional activation of BDNF. (A and B) BDNF expression in HeLa clonal cell lines containing the indicated expression vectors (HBZ-S1 wild type and mutants and HBZ-US wild type). The graphs show results from real-time PCR amplification of BDNF normalized to amplification of UBE2D2. Values correspond to three independent experiments and were calculated relative to one data set for cells with pcDNA3.1 alone. Error bars show standard deviations. *, P < 0.05; **, P < 0.005 (two-tailed Student t test). Lower panels show Western blots of whole-cell extracts prepared from cell lines corresponding to those listed above. Membranes were probed with a Myc antibody for detection of epitope-tagged HBZ and then stripped and probed for actin. ns, nonspecific band.