FIG 2.

MNK inhibition reduces PVSRIPO translation, proliferation, and cytotoxicity in GBM cells. (A) Schema of signaling pathways to translation initiation factors investigated in this study. (B) U87, Du54, and 43 GBM cell lines were pretreated (1 h) with DMSO or CGP57380 (10 or 30 μM), followed by infection in the presence of DMSO/CGP57380. Cells were harvested (6 h p.i.), and viral protein (2C) was assessed by immunoblotting. Supernatants were collected (12 h p.i.) to determine viral progeny and ATP release, as indicated. The bars represent the average of 3 experiments normalized to the control (DMSO) values, and the error bars represent SEM. The asterisks denote significant ANOVA-protected t tests.