FIG 8.

MNK1 depletion causes SRPK nuclear accumulation and SF2 nuclear-speckle dissociation. (A) Whole-cell, cytoplasmic, and nuclear lysates were prepared from cells treated with siCtrl with and without TPA (1 h) or siMNK plus TPA (1 h). Immunoblots for SRPK2 were quantitated, and average SRPK2 values for each fraction from two experiments are shown; the asterisk denotes an ANOVA-protected t test. The error bars indicate SEM. (B to U) Indirect IF using cells treated with siCtrl (with or without TPA; 1 h), siMNK (plus TPA; 1 h), or siSRPK1 were stained for SRPK1 (green), nuclear-speckle marker SF2 (red), and DAPI (blue), as labeled. Individual staining for each group (B to Q) and merged tricolor staining (R to U) are shown. A negative-staining (no primary antibody added) control for SRPK1 and SF2 is shown on the right (F, L, and Q). The indirect IF experiment was performed three times, and representative images are shown.