Phylogenetic analysis of envelope sequences amplified from virus stocks used in the in vivo passage, SIVsmH445, SIVsmH631Br, SIVsmH783Br, and SIVsmH804E. Shown is a phylogenetic analysis of envelope nucleotide sequences. The black letters indicates the original SIVsmE543-3, purple indicates the SIVsmH445 variants, green indicates the SIVsm631Br isolate, red indicates the SIVsm783Br isolate, and blue indicates the final isolate, SIVsm804E.