FIG 1.

Growth curves of single-clade infections in Aag2 mosquito cells. Equal numbers of GE of five viral isolates from each clade were used to infect Aag2 cells, and supernatants were collected on days 2 to 6 postinfection. Supernatants were analyzed by determination of the number of focus-forming units on BHK cell monolayers, using anti-DENV monoclonal antibody 4G2. Plotted are the mean and standard error for the isolates at each time point. An unpaired t test revealed no statistically significant difference between the clades on any given day: day 2, P > 0.999; day 3, P = 0.318; day 4, P = 0.532; day 5, P = 0.944; and day 6, P = 0.667.