FIG 4.

Titer and infectivity of DENV-2 clades in single infections in A. aegypti mosquitoes. (A) Titer (GE) of NI-1 or NI-2B viruses in Nicaraguan A. aegypti mosquito tissues on day 7 in single infections. Statistical significance was determined by the Mann-Whitney unpaired t test. P value for carcasses, 0.7379; P value for legs, 0.2081. (B) Infectivity calculated from the number of infected mosquitoes in a group (determined by RT-PCR) divided by the number of blood-fed mosquitoes in the same group. A two-sided Z-test comparing the proportions of the two groups was used to determine significance: for carcasses, P = 0.841; for legs, P = 0.575.