FIG 8.

Minocycline (Mino) treatment alters cytokine/chemokine gene expression in WNV-infected SCSCs and minimally reduces the viral titer. WNV-infected SCSC samples were treated with either minocycline (50 μM) or vehicle (medium). Samples were collected at 1 dpi, 3 dpi, 5 dpi, and 7 dpi (n = 3), and mRNA was isolated for RT-qPCR analysis. (A) The levels of cytokine/chemokine mRNA expression were normalized to the level of β-actin mRNA expression for each time point and are depicted as relative fold increases. Each bar represents the mean level of expression. (B) The viral load (numbers of PFU equivalents) was determined using a standard curve created from RNA from WNV-infected brain tissue. The viral load is measured on a log₁₀ scale. Error bars represent the standard errors of the means. Asterisks indicate values that are statistically significant (***, P < 0.001; **, P < 0.005; *, P < 0.05).