FIG 2.
Plasma IgG binding affinity to autologous Envs. The plasma IgG affinity to autologous Envs was determined by Octet biolayer interferometry (BLI). (A and B) The Env gp140 proteins used in this assay were derived from the early env sequences 014_04/15/04_F8 (A) and 013_9/23/04_C1 (B). The affinities of longitudinal IgG samples from subjects VC10014 (A) and VC20013 (B) were determined and plotted over time. The affinity measurements reported here are the equilibrium dissociation constants for each sample (KD), which were determined at multiple concentrations in replicate assays. YPI, year postinfection.