FIG 8.

VC10014 epitope mapping in the autologous isolates. We tested the effects of mutations within the C2 region of Env and insertions and deletions in the V4 region of Env on the neutralization phenotype of autologous viruses isolated from subject VC10014. (A) Effects of potential escape mutations on neutralization sensitivity of early isolates to early or contemporaneously matched plasma. We tested variants in which we introduced F277I/N279D or the V4 insertion motif SSWN and variants with both the mutation and insertion (F277I/N279D/SSWN). (B) Effects of potential reversion mutations in the later, neutralization-resistant isolates against late or contemporaneously matched plasma. The reversion mutations include I277F/D279N, ΔSSWN, or ΔDTST and I277F/D279N/ΔSSWN or I277F/D279N/ΔDTST. YPI, years postinfection; wt, wild type; ΔSSWN or ΔDTST, deletion of the four amino acids; FI/ND/ΔDTST or FI/ND/ΔSSWN, I277F, D279N, and deletion of SSWN or DTST in the V4.