FIG 2.

Coimmunoprecipitation of Nipah virus M protein with candidate host proteins identified by MS. 293T cells were transfected to produce Myc-tagged Nipah virus M protein together with the indicated Flag-tagged candidate host factors. Proteins synthesized in the transfected cells were metabolically labeled, and cells were lysed in a solution containing 1% NP-40. Immunoprecipitation was carried out using Myc antibody (lane 1) or anti-Flag conjugated resin (lanes 2 to 9), and proteins were detected using a phosphorimager.