FIG 3.

Self-oligomerization of wild-type M and the C-terminal truncation mutations. A plasmid encoding HA-M was transfected singly or cotransfected with the indicated plasmids encoding Myc-tagged M or mutants into 293T cells. At 48 h posttransfection, the cells were harvested and subjected to coimmunoprecipitation assay. Immunoprecipitation (IP) of the whole-cell lysates was done with anti-Myc polyclonal Ab. WB analysis of lysates and precipitated proteins was done with either anti-HA or anti-Myc monoclonal Ab. The bands marked by stars represent detected proteins. Coprecipitated HA-M of each lane was quantified, and standard errors were calculated from two independent experiments.