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VLP budding of L302A in the presence of other virus proteins. 293T cells were transfected with the indicated plasmids for 48 h, and the VLP budding assay was performed as described in the legend to Fig. 1. WB was performed with anti-HA, anti-Myc, anti-Flag, and anti-His Abs. Quantification of the budding index for each group is shown. Standard errors were calculated from three independent experiments.
