FIG 4.

Mucosal and serum IgG binding to linear Env peptides by peptide microarrays. IgG binding to 3,882 linear Env peptides was assessed by peptide microarrays using serum and mucosal secretions from 5 monkeys immunized with the Env protein vaccine 16 weeks after the final immunization as well as 2 naive control monkeys. (A) Mean signal intensity of binding is plotted by the peptide gp140 start position for serum. (B) Mean signal intensity of binding is plotted for mucosal samples. (C) Mean signal intensity of mucosal peptide binding is plotted against mean signal intensity of serum peptide binding for the 5 immunized monkeys. (D) The breadth (number of binding sites) and depth (median number of epitope variants/binding site) for each immunized monkey are plotted for serum and mucosal samples. Mean responses and standard errors of the means (SEM) are depicted.