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Primer pairs and Tms used in the conventional PCR to check the presence of in silico-determined Sau3AI sequences from the key genes in the ASFVUblib
| ASFV gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Tm (°C) |
|---|---|---|---|
| B646L | CCTCAAACCCCTAAATACT | ATCGGAGATGTTCCAGGTA | 56 |
| A179L | ATCACTACGGCATACAACT | TAACTGTACACAGGATCTG | 54 |
| A224L | GATGCACGAAATCAAAGCT | AATGATCTTATGAATGTATTTTC | 54 |
| G1340L | CAGGTCTGGGCGTTATAGA | TTTTACACTAATAATTTCCTG | 56 |
| I329L | GATTATAACATACTCAGAAAAC | ATATTTTTTACAAATAGAACGC | 54 |
