FIG 1.

Type I IFN receptor-deficient mice have increased numbers of MHV68-specific CD8⁺ T cells that are skewed toward a short-lived effector phenotype. WT or IFNAR1^−/− mice were infected with MHV68, and splenocytes were harvested at the time points indicated. (A and B) Splenocytes were labeled with anti-CD8 (α-CD8), α-CD44, and either D^bp56 (p56) or K^bp79 (p79) MHC class I tetramers, and numbers of tetramer-positive CD8⁺ CD44^hi cells were calculated. Representative dot plots with the percentage of parent indicated in the quadrants (A) and data from the indicated times postinfection (B) are shown. (C and D) Splenocytes were labeled with α-CD8, α-KLRG-1, α-CD127, and either p56 or p79 tetramers, and numbers of SLECs were calculated. Representative dot plots (C) and data from the indicated times postinfection (D) are shown. Means ± standard errors of the mean (SEM), representing five to nine mice at each time point, are shown. Horizontal dotted line in panels B and D indicates background in naive mice as gated; #, number of. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (paired, two-tailed Student's t test).