FIG 3.

The defect in coproduction of TNF-α and IFN-γ in IFNAR1^−/− CD8⁺ T cells is uniform among effector differentiation subtypes. WT and IFNAR1^−/− mice were infected with MHV68 and euthanized at 16 dpi. Splenocytes were stimulated with p79 peptide and labeled with α-CD8, α-KLRG-1, α-CD127, α-IFN-γ, and α-TNF-α and evaluated by flow cytometry. Bars represent percent IFN-γ⁺ CD8⁺ T cells also producing TNF-α following peptide stimulation. Means ± SEM, representing six total mice per group from two independent experiments, are shown. **, P ≤ 0.01; ***, P ≤ 0.001 (paired, two-tailed Student's t test). SLEC, short-lived effector cells (KLRG-1⁺ CD127⁻); DPEC, double-positive effector cells (KLRG-1⁺ CD127⁺); MPEC, memory precursor effector cells (KLRG-1⁻ CD127⁺); EEC, early effector cells (KLRG-1⁻ CD127⁻).