FIG 9.

Immunogold labeling of core proteins. Cells were infected with WR or Ets85 at an MOI of 10 and incubated at 31°C or 39.7°C. At 24 h postinfection, cells were processed for immunoelectron microscopy. Ultrathin sections were probed with antibodies against L4 (A to D) or A3 (E to H) and visualized in an electron microscope (Hitachi H-7000). (A and E) WR at 31°C. (B and F) WR at 40°C. (C and G) Ets85 at 31°C. (D and H) Ets85 at 39.7°C. The insets show the labeling of immature virions. Scale bar, 500 nm.