FIG 7.

gp130 expression during KSHV infection is required to downregulate TGF-β2. (A) qRT-PCR analysis of TGF-β2 mRNA from TIME cells transduced with either scrambled siRNA or siRNA against gp130 and subsequently mock infected or infected with KSHV BAC16_WT or KSHV BAC16_ΔmiR. Cells were harvested, and total RNA was isolated and analyzed by quantitative real-time RT-PCR. Values for samples were normalized to GAPDH values and are reported as fold changes over values for mock-infected cells. Error bars reflect standard errors of the means of data from three independent experiments (*, P < 0.05). (B) qRT-PCR analysis of gp130 from TIME cells treated with scrambled siRNA and mock infected or infected with KSHV BAC16_WT or KSHV BAC16_ΔmiR performed as described above for panel A.