FIG 3.

Human adenovirus E4-ORF1 proteins promote Dlg1-dependent PI3K activation in both stable E4-ORF1-expressing and adenovirus-infected cells. (A) Extracts of vector cells or the indicated HA-E4-ORF1 lines transduced with either the Dlg1 shRNA (+) or the control scrambled shRNA (−) were analyzed in immunoblot assays. (B) Vector cells transduced with either the Dlg1 shRNA (+) or the control scrambled shRNA (−) were mock infected or infected with the indicated wt adenovirus at an MOI of 1. At 24 hpi, cell extracts were prepared and analyzed in immunoblot assays.