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Human adenovirus E4-ORF1 proteins induce PI3K-dependent cellular transformation. The indicated MCF10A lines treated with either a DMSO vehicle (−) or 100 μM LY294002 (LY) (+) were analyzed in soft-agar assays as described in Materials and Methods. LY treatment began 2 days postplating. Cells were photographed at 11 days postplating (magnification, ×40).
