FIG 5.

Human adenovirus E4-ORF1 proteins also form the ternary complex during viral infections. (A) MCF10A cells were mock infected or infected with the indicated wt adenovirus at an MOI of 1. Extracts (300 μg of protein) prepared at 24 hpi were immunoprecipitated with Dlg1 antibody. Recovered proteins, as well as cell extract (input), were analyzed in immunoblot assays. (B) MCF10A cells either mock infected or infected with the indicated wt adenovirus at an MOI of 1 show either no cytopathic effect at 6 hpi or virus-specific cytopathic effect at 24 hpi. Cells were visualized by phase-contrast microscopy (magnification, ×40). (C) MCF10A cells either mock infected or infected with the indicated wt adenovirus at an MOI of 1 accumulate either undetectable amounts or similar amounts of the major capsid protein hexon at 6 hpi or 24 hpi, respectively. Cell extracts were resolved by SDS-PAGE, and the gel was stained with Coomassie brilliant blue.